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Retinal Degeneration pp 257–270Cite as

Ca2+ Microfluorimetry in Retinal Müller Glial Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 935))

Abstract

Calcium acts as a prominent second messenger in virtually every cell type and modulates a plethora of cell functions. Thus, Ca2+ microfluorimetry became a valuable tool to assess information about mechanisms involved in the regulation of the intracellular calcium level in research on living tissues. Here we offer insight into distinct approaches to detect changes in calcium levels specifically in Müller cells, the principal macroglial cells of the retina.

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Acknowledgments

This work was supported by the Deutsche Forschungsgesellschaft (SPP 1172: T.P., A.R.; FOR748: A.W., A.R.; GRK 1097: A.R.; PA 615/2-1). The authors are indebted to Jens Grosche (effigos AG, www.effigos.de) for professional assistance with the illustrations.

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Authors and Affiliations

  1. Paul Flechsig Institute for Brain Research, Department of Pathophysiology of Neuroglia, University of Leipzig, Leipzig, Germany

    Antje Wurm, Thomas Pannicke & Andreas Reichenbach

Authors
  1. Antje Wurm
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  2. Thomas Pannicke
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  3. Andreas Reichenbach
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Corresponding author

Correspondence to Andreas Reichenbach .

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Editors and Affiliations

  1. , Institute of Human Genetics, University of Regensburg, Franz-Josef-Strauss-Allee 11, Regensburg, 93053, Germany

    Bernhard H.F. Weber

  2. INSTITUTE OF HUMAN GENETICS, INSTITUTE OF HUMAN GENETICS, FRANZ-JOSEF-STRAUSS-ALLEE 11, 93053 REGENSBURG, Germany

    THOMAS LANGMANN

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Wurm, A., Pannicke, T., Reichenbach, A. (2012). Ca2+ Microfluorimetry in Retinal Müller Glial Cells. In: Weber, B., LANGMANN, T. (eds) Retinal Degeneration. Methods in Molecular Biology, vol 935. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-080-9_18

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  • DOI: https://doi.org/10.1007/978-1-62703-080-9_18

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-079-3

  • Online ISBN: 978-1-62703-080-9

  • eBook Packages: Springer Protocols

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