Abstract
Sporozoites, the stage of Plasmodium infectious to vertebrates when injected in the skin by a mosquito vector, are highly motile cells. Their unusual form of gliding motility is essential for infectivity, allowing the parasite to travel through both the mosquito and mammalian hosts, invading different cell types and escaping immune cell-mediated death. In this chapter, we describe techniques to study gliding motility of sporozoites in vitro and in vivo.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Heintzelman MB (2006) Cellular and molecular mechanics of gliding locomotion in eukaryotes. Int Rev Cytol 251:79–129
Munter S et al (2009) Plasmodium sporozoite motility is modulated by the turnover of discrete adhesion sites. Cell Host Microbe 6:551–562
Stewart MJ, Vanderberg JP (1998) Malaria sporozoites leave behind trails of circumsporozoite protein during gliding motility. J Protozool 35:389–393
Vanderberg JP (1974) Studies on the motility of Plasmodium sporozoites. J Protozool 21:527–537
Frevert U et al (2005) Intravital observation of Plasmodium berghei sporozoite infection of the liver. PLoS Biol 3:e192
Tardieux I, Menard R (2008) Migration of Apicomplexa across biological barriers: the Toxoplasma and Plasmodium rides. Traffic 9:627–635
Vanderberg JP, Frevert U (2004) Intravital microscopy demonstrating antibody-mediated immobilisation of Plasmodium berghei sporozoites injected into skin by mosquitoes. Int J Parasitol 34:991–996
Matuschewski K (2006) Getting infectious: formation and maturation of Plasmodium sporozoites in the Anopheles vector. Cell Microbiol 8:1547–1556
Amino R et al (2006) Quantitative imaging of Plasmodium transmission from mosquito to mammal. Nat Med 12:220–224
Mota MM et al (2001) Migration of Plasmodium sporozoites through cells before infection. Science 291:141–144
Kebaier C, Vanderberg JP (2010) Initiation of Plasmodium sporozoite motility by albumin is associated with induction of intracellular signalling. Int J Parasitol 40:25–33
Hellmann JK et al (2011) Environmental constraints guide migration of malaria parasites during transmission. PLoS Pathog 7:e1002080
Hegge S et al (2009) Automated classification of Plasmodium sporozoite movement patterns reveals a shift towards productive motility during salivary gland infection. Biotechnol J 4:903–913
Acknowledgments
We thank Pauline Formaglio and Laura Schnettger for reading and discussing the manuscript.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Carey, A.F., Ménard, R., Bargieri, D.Y. (2012). Scoring Sporozoite Motility. In: Ménard, R. (eds) Malaria. Methods in Molecular Biology, vol 923. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-026-7_26
Download citation
DOI: https://doi.org/10.1007/978-1-62703-026-7_26
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-025-0
Online ISBN: 978-1-62703-026-7
eBook Packages: Springer Protocols