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Adoptive Transfer of Splenocytes to Immunocompromised Mice

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Helicobacter Species

Part of the book series: Methods in Molecular Biology ((MIMB,volume 921))

Abstract

Analysis of the immune response of mice to Helicobacter infection has been greatly aided by the use of various deficient mouse strains. Here we present protocols for reconstitution of immune-deficient mice with wild-type immune cells and protocols for analysis of the outcome.

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References

  1. Eaton KA, Ringler SR, Danon SJ (1999) Murine splenocytes induce severe gastritis and delayed-type hypersensitivity and suppress bacterial colonization in Helicobacter pylori-infected SCID mice. Infect Immun 67:4594–4602

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  2. Eaton KA, Mefford M, Thevenot T (2001) The role of T cell subsets and cytokines in the pathogenesis of Helicobacter pylori gastritis in mice. J Immunol 166:7456–7461

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  3. Eaton KA, Mefford ME (2001) Cure of Helicobacter pylori infection and resolution of gastritis by adoptive transfer of splenocytes in mice. Infect Immun 69:1025–1031

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Correspondence to Kathryn A. Eaton .

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© 2012 Springer Science+Business Media, LLC

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Gray, B.M., Eaton, K.A. (2012). Adoptive Transfer of Splenocytes to Immunocompromised Mice. In: Houghton, J. (eds) Helicobacter Species. Methods in Molecular Biology, vol 921. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-005-2_14

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  • DOI: https://doi.org/10.1007/978-1-62703-005-2_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-004-5

  • Online ISBN: 978-1-62703-005-2

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