Abstract
Computer analysis of digital photographic images provides fast, high-throughput screening of leaf pigmentation. Pixel-by-pixel conversion of red, green, blue (RGB) parameters to hue, saturation, value (HSV) showed that Hue values were proportional to total chlorophyll, offering an alternative to photometric analysis of leaf extracts. This is demonstrated using tobacco leaves with various chlorophyll contents due to senescence but shows the possibility of applications in studies of stress conditions accompanied by chlorophyll loss.
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Sass, L., Majer, P., Hideg, É. (2012). Leaf Hue Measurements: A High-Throughput Screening of Chlorophyll Content. In: Normanly, J. (eds) High-Throughput Phenotyping in Plants. Methods in Molecular Biology, vol 918. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-995-2_6
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DOI: https://doi.org/10.1007/978-1-61779-995-2_6
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Publisher Name: Humana Press, Totowa, NJ
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