Abstract
Lignocellulosic biomass is a source of low cost polysaccharides that some microbes can deconstruct and convert into liquid transportation fuel. Feedstocks vary in their ease of use depending on their source and handing. Estimating conversion amenability is useful to determine the effects of biomass pretreatment and genetic potential for the purposes of energy crop breeding and genetics. Here we describe a small-scale high-throughput assay that measures ethanol production from a culture of plant biomass and the ethanologen Clostridium phytofermentans.
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Acknowledgments
This research was supported by the Office of Science (BER) Department of Energy Grant DE-FG02-08ER64700DE to SPH. We thank Michael Keedy and Eric Bertrand (Biology Department, University of Massachusetts Amherst) for design and construction of the 96-well plate autoclave clamp.
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Lee, S.J., Warnick, T.A., Leschine, S.B., Hazen, S.P. (2012). A High-Throughput Biological Conversion Assay for Determining Lignocellulosic Quality. In: Normanly, J. (eds) High-Throughput Phenotyping in Plants. Methods in Molecular Biology, vol 918. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-995-2_18
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DOI: https://doi.org/10.1007/978-1-61779-995-2_18
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