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Therapeutic Proteins pp 265–275Cite as

Methods for Chromatographic Removal of Endotoxin

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 899))

Abstract

Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method for endotoxin removal at the pilot production scale using fast protein liquid chromatography and buffers specifically engineered for endotoxin removal.

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Author information

Authors and Affiliations

  1. Graduate Field of Microbiology, Cornell University, Ithaca, NY, USA

    Adam J. Lowe

  2. SRC Inc, Syracuse, NY, USA

    Adam J. Lowe

  3. Biomedical Engineering, Cornell University, Ithaca, NY, USA

    Cameron L. Bardliving

  4. Department of Food Science, Cornell University, Ithaca, NY, USA

    Carl A. Batt

Authors
  1. Adam J. Lowe
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  2. Cameron L. Bardliving
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  3. Carl A. Batt
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Corresponding author

Correspondence to Adam J. Lowe .

Editor information

Editors and Affiliations

  1. MedImmune, LLC, MedImmune Way One, Gaithersburg, 20878, USA

    Vladimir Voynov

  2. , Department of Physical Biochemistry, Biogen Idec, Cambridge Center 14, Cambridge, 02142, Massachusetts, USA

    Justin A. Caravella

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© 2012 Springer Science+Business Media, LLC

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Cite this protocol

Lowe, A.J., Bardliving, C.L., Batt, C.A. (2012). Methods for Chromatographic Removal of Endotoxin. In: Voynov, V., Caravella, J. (eds) Therapeutic Proteins. Methods in Molecular Biology, vol 899. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-921-1_17

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  • DOI: https://doi.org/10.1007/978-1-61779-921-1_17

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-920-4

  • Online ISBN: 978-1-61779-921-1

  • eBook Packages: Springer Protocols

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