Abstract
In recent years, in situ RNA hybridization technique has found a widespread application in developmental biology. This method has frequently been used to determine gene expression patterns, which is a first step toward understanding of a gene function. Here, we provide a reliable and sensitive method for in situ RNA hybridization on frozen sections of mouse embryo using digoxigenin-labeled RNA probes. This technique can be used to study gene expression patterns at all stages of odontogenesis.
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Kyrylkova, K., Kyryachenko, S., Kioussi, C., Leid, M. (2012). Determination of Gene Expression Patterns by In Situ Hybridization in Sections. In: Kioussi, C. (eds) Odontogenesis. Methods in Molecular Biology, vol 887. Humana Press. https://doi.org/10.1007/978-1-61779-860-3_3
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DOI: https://doi.org/10.1007/978-1-61779-860-3_3
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Online ISBN: 978-1-61779-860-3
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