Abstract
The development of near isogenic lines (NILs) through repeated backcrossing of genetically distinct parental lines is rather straightforward. Nonetheless, depending on the available resources and the purpose of the lines to be generated, several choices can be made to guide the design of such inbred populations. Here we outline the implications of these choices and provide recommendations for the efficient and proper development of NILs for a number of common scenarios.
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References
Eshed Y, Zamir D (1995) An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics 141:1147–1162
Alonso-Blanco C, Bentsink L, Hanhart CJ, Vries HBE, Koornneef M (2003) Analysis of natural allelic variation at seed dormancy loci of Arabidopsis thaliana. Genetics 164:711–729
Tuinstra MR, Ejeta G, Goldsbrough PB (1997) Heterogeneous inbred family (HIF) analysis: a method for developing near-isogenic lines that differ at quantitative trait loci. Theor Appl Genet 95:1005–1011
Hospital F (2002) Marker-assisted backcross breeding: a case study in genotype building theory. In: Kang MS (ed) Quantitative genetics, genomics and plant breeding. CAB International, New York
Loudet O, Gaudon V, Trubuil A, Daniel-Vedele F (2005) Quantitative trait loci controlling root growth and architecture in Arabidopsis thaliana confirmed by heterogeneous inbred family. Theor Appl Genet 110:742–753
Keurentjes JJB, Bentsink L, Alonso-Blanco C, Hanhart CJ, Blankestijn-De Vries H, Effgen S et al (2007) Development of a near-isogenic line population of Arabidopsis thaliana and comparison of mapping power with a recombinant inbred line population. Genetics 175:891–905
Frisch M, Bohn M, Melchinger AE (1999) Comparison of selection strategies for marker-assisted backcrossing of a gene. Crop Sci 39:1295–1301
Falke KC, Miedaner T, Frisch M (2009) Selection strategies for the development of rye introgression libraries. Theor Appl Genet 119:595–603
Koumproglou R, Wilkes TM, Townson P, Wang XY, Beynon J, Pooni HS et al (2002) STAIRS: a new genetic resource for functional genomic studies of Arabidopsis. Plant J 31:355–364
Allard RW (1960) Principles of plant breeding. Wiley, New York
Nadeau JH, Singer JB, Matin A, Lander ES (2000) Analysing complex genetic traits with chromosome substitution strains. Nat Genet 24:221–225
Visscher PM, Haley CS, Thompson R (1996) Marker-assisted introgression in backcross breeding programs. Genetics 144:1923–1932
Frisch M, Bohn M, Melchinger AE (1999) Minimum sample size and optimal positioning of flanking markers in marker-assisted backcrossing for transfer of a target gene. Crop Sci 39:967–975
Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12:368–375
Canady MA, Ji Y, Chetelat RT (2006) Homeologous recombination in Solanum lycopersicoides introgression lines of cultivated tomato. Genetics 174:1775–1788
Wijnker E, de Jong H (2008) Managing meiotic recombination in plant breeding. Trends Plant Sci 13:640–646
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Kooke, R., Wijnker, E., Keurentjes, J.J.B. (2012). Backcross Populations and Near Isogenic Lines. In: Rifkin, S. (eds) Quantitative Trait Loci (QTL). Methods in Molecular Biology, vol 871. Humana Press. https://doi.org/10.1007/978-1-61779-785-9_1
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DOI: https://doi.org/10.1007/978-1-61779-785-9_1
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