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DNA Barcoding Methods for Invertebrates

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DNA Barcodes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 858))

Abstract

Invertebrates comprise approximately 34 phyla, while vertebrates represent one subphylum and insects a (very large) class. Thus, the clades excepting vertebrates and insects encompass almost all of animal diversity. Consequently, the barcoding challenge in invertebrates is that of barcoding animals in general. While standard extraction, cleaning, PCR methods, and universal primers work for many taxa, taxon-specific challenges arise because of the shear genetic and biochemical diversity present across the kingdom, and because problems arising as a result of this diversity, and solutions to them, are still poorly characterized for many metazoan clades. The objective of this chapter is to emphasize general approaches, and give practical advice for overcoming the diverse challenges that may be encountered across animal taxa, but we stop short of providing an exhaustive inventory. Rather, we encourage researchers, especially those working on poorly studied taxa, to carefully consider methodological issues presented below, when standard approaches perform poorly.

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Authors and Affiliations

  1. Florida Museum of Natural History, University of Florida, Gainesville, FL, USA

    Nathaniel Evans & Gustav Paulay

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  1. Nathaniel Evans
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  2. Gustav Paulay
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Correspondence to Gustav Paulay .

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  1. National Museum of Natural History, Dept. Botany, Smithsonian Institution, 10th St. & Constitution Ave. NW.,, Washington, 20560-0166, District of Columbia, USA

    W. John Kress

  2. National Museum of Natural History, Dept. Botany, Smithsonian Institution, 10th St. & Constitution Ave. NW.,, Washington, 20560-0166, District of Columbia, USA

    David L. Erickson

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Evans, N., Paulay, G. (2012). DNA Barcoding Methods for Invertebrates. In: Kress, W., Erickson, D. (eds) DNA Barcodes. Methods in Molecular Biology, vol 858. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-591-6_4

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  • DOI: https://doi.org/10.1007/978-1-61779-591-6_4

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