Abstract
In vitro culture of mammalian cells is fundamental to various biological studies such as single cell analysis, pathological research, and drug/therapy development. However, there are limitations with the current in vitro cell culture methods. Cells tend to lose their specific functions due to the lack of a cellular microenvironment when they are maintained under standard culture conditions. Microscale devices can be a novel tool to reestablish a cellular microenvironment for culturing mammalian cells in vitro and maintaining their differentiated functions. Different microscale cell culture techniques have been developed to suit different biological applications. This chapter describes a method to trap and culture mammalian cells in a microfluidic channel with a built-in pillar array.
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Zhang, C. (2012). Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Built-In Pillar Array. In: Lindström, S., Andersson-Svahn, H. (eds) Single-Cell Analysis. Methods in Molecular Biology, vol 853. Humana Press. https://doi.org/10.1007/978-1-61779-567-1_8
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DOI: https://doi.org/10.1007/978-1-61779-567-1_8
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