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Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans

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Host-Fungus Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 845))

Abstract

Techniques used to generate mutants in Candida albicans commonly result in additional and undesired genetic rearrangements. Detection of aneuploidy is, therefore, an important step forward in the quality control of mutant phenotypes. In this chapter, we describe how to extract genomic DNA and perform a quantitative multiplex PCR to compare the karyotype of any mutant strain to that of its parent and allow the detection of any unwanted aneuploidy.

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Acknowledgments

M. Lenardon is supported by a project grant from the Wellcome Trust (086827).

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Authors and Affiliations

  1. School of Medical Sciences, University of Aberdeen, Aberdeen, UK

    Megan D. Lenardon

  2. Biotechnology Research Institute, National Research Council of Canada, Montreal, QC, Canada

    André Nantel

Authors
  1. Megan D. Lenardon
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  2. André Nantel
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Corresponding author

Correspondence to Megan D. Lenardon .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Alexandra C. Brand

  2. Institute of Medical Sciences, School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Donna M. MacCallum

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© 2012 Springer Science+Business Media, LLC

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Lenardon, M.D., Nantel, A. (2012). Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans . In: Brand, A., MacCallum, D. (eds) Host-Fungus Interactions. Methods in Molecular Biology, vol 845. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-539-8_3

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  • DOI: https://doi.org/10.1007/978-1-61779-539-8_3

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  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-538-1

  • Online ISBN: 978-1-61779-539-8

  • eBook Packages: Springer Protocols

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