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Mini-blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans

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Host-Fungus Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 845))

Abstract

Several gene disruption strategies have been described in Candida albicans to create homozygous mutants. We describe here a recyclable mini-blaster cassette containing C. albicans URA3 gene and 200-bp flanking repeats that is useful for disruption of C. albicans genes. The cassette can be used to create unmarked homozygous mutants which can be complemented at the HIS1 gene locus. This strategy of creating gene disruptions and subsequent complementation can be used to study gene function.

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Acknowledgments

This work was supported by NIH grant R01 AI067703 to APM. We thank Dr. Carol A. Woolford for her comments on this chapter.

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Authors and Affiliations

  1. Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA

    Shantanu Ganguly & Aaron P. Mitchell

Authors
  1. Shantanu Ganguly
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  2. Aaron P. Mitchell
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Corresponding author

Correspondence to Aaron P. Mitchell .

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Editors and Affiliations

  1. Institute of Medical Sciences, School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Alexandra C. Brand

  2. Institute of Medical Sciences, School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Donna M. MacCallum

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Ganguly, S., Mitchell, A.P. (2012). Mini-blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans . In: Brand, A., MacCallum, D. (eds) Host-Fungus Interactions. Methods in Molecular Biology, vol 845. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-539-8_2

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  • DOI: https://doi.org/10.1007/978-1-61779-539-8_2

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  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-538-1

  • Online ISBN: 978-1-61779-539-8

  • eBook Packages: Springer Protocols

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