Abstract
Evaluating endocrine activities of environmental chemicals or screening for new small molecule modulators of the androgen receptor (AR) transcription activity requires standardized and reliable assay procedures. Scintillation proximity assays (SPA) are sensitive and reliable techniques that are suitable for ligand competition binding assays. We have utilized a radiolabeled ligand competition binding assay for the androgen receptor (AR) that can be carried out in a 384-well format. This standardized, highly reproducible and low-cost assay has been automated for high-throughput screening (HTS) purposes.
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Acknowledgments
This work was supported by the American Lebanese Syrian Associated Charities (ALSAC), St. Jude Children’s Research Hospital (SJCRH), the NIH (DK58080) and the Department of Defense Prostate Cancer Research Program (PC060344-W81XWH-07-1-0073).
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Féau, C., Arnold, L.A., Kosinski, A., Guy, R.K. (2011). Ligand Competition Binding Assay for the Androgen Receptor. In: Saatcioglu, F. (eds) Androgen Action. Methods in Molecular Biology, vol 776. Humana Press. https://doi.org/10.1007/978-1-61779-243-4_4
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DOI: https://doi.org/10.1007/978-1-61779-243-4_4
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