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Tissue-Specific Knockout of Androgen Receptor in Mice

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Book cover Androgen Action

Part of the book series: Methods in Molecular Biology ((MIMB,volume 776))

Abstract

Androgen acting through the androgen receptor (AR) is known to be essential for male sexual differentiation and development. Using Cre-lox technology, we have generated the floxed AR mice, which have been bred with general or tissue-specific Cre expressing transgenic mice to knock out the AR gene in specific target cells. Our findings indicated that AR is required for sexual development and that loss of AR can have significant effects on many aspects of physiological functions and disease progression, such as immune function, metabolism, and tumorigenesis. Furthermore, our strategy can generate AR knockout (ARKO) in female mice, which allows researchers to study the AR function in the female. In brief, our floxed AR mouse model provides a powerful tool to study in vivo AR functions in selective tissues and cell types and has made possible several research breakthroughs in the field of endocrinology.

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Acknowledgments

The authors would like to thank Karen Wolf for manuscript preparation. This work was supported by RO1 CA127300 and the George H. Whipple Professorship Endowment.

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Authors and Affiliations

  1. George H. Whipple Lab, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY, 14642-8626, USA

    Tzu-hua Lin, Shuyuan Yeh & Chawnshang Chang

Authors
  1. Tzu-hua Lin
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  2. Shuyuan Yeh
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  3. Chawnshang Chang
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Corresponding author

Correspondence to Chawnshang Chang .

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Editors and Affiliations

  1. Dept. Molecular Biosciences, University of Oslo, Oslo, Norway

    Fahri Saatcioglu

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Lin, Th., Yeh, S., Chang, C. (2011). Tissue-Specific Knockout of Androgen Receptor in Mice. In: Saatcioglu, F. (eds) Androgen Action. Methods in Molecular Biology, vol 776. Humana Press. https://doi.org/10.1007/978-1-61779-243-4_16

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  • DOI: https://doi.org/10.1007/978-1-61779-243-4_16

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-242-7

  • Online ISBN: 978-1-61779-243-4

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