Abstract
Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurring organellar DNA insertions – essentially any unique sequence larger than 2 kb – greatly facilitates progress. This chapter details the labeling procedures and chromosome preparation techniques used to produce high-quality FISH signals on somatic metaphase and meiotic pachytene spreads.
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Acknowledgments
This work was supported by grants from the National Science Foundation DBI 0423898, DBI 0421671 and DBI 0701297.
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Kato, A. et al. (2011). Chromosome Painting for Plant Biotechnology. In: Birchler, J. (eds) Plant Chromosome Engineering. Methods in Molecular Biology, vol 701. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-957-4_4
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DOI: https://doi.org/10.1007/978-1-61737-957-4_4
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