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Identifying Chloroplast Biogenesis and Signalling Mutants in Arabidopsis thaliana

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Photosynthesis Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 684))

Abstract

The chloroplast is the largest and arguably the most complex of the three energy organelles in the plant cell. The biogenesis of the chloroplast requires a combination of thousands of proteins encoded by the chloroplastic and nuclear genomes. Chloroplast function is also subject to modifications to enable responses to changes in environmental and developmental stimuli. As a consequence, interorganelle signalling and coordination between the chloroplast and nucleus is critical for the biogenesis and function of the chloroplast. Coordination and signalling during biogenesis is referred to as biogenic control and during the function as operational control (1). In this article, we report on two different mutant screens as examples of strategies for identifying mutations that affect biogenic and operational control signalling pathways and processes. We also describe strategies for the analysis and genotyping of the mutants.

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Acknowledgments

We would like to thank Dr. Phil Mullineaux (John Innes Institute, Norwich, UK) for kindly providing us with EMS-mutagenised APX2-luciferase seed. We also thank Jan Bart Rossel (Australian National University) for input into the first version of this manuscript.

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Authors and Affiliations

  1. School of Biochemistry and Molecular Biology, The Australian National University, Canberra, ACT, Australia

    Verónica Albrecht, Gonzalo M. Estavillo, Abby J. Cuttriss & Barry J. Pogson

Authors
  1. Verónica Albrecht
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  2. Gonzalo M. Estavillo
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  3. Abby J. Cuttriss
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  4. Barry J. Pogson
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  1. , Département de Chimie Biologie, Université du Québec à Trois-Rivières, Boul. des Forges 3351, Trois-Rivieres, G9A5H7, Québec, Canada

    Robert Carpentier

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Albrecht, V., Estavillo, G.M., Cuttriss, A.J., Pogson, B.J. (2011). Identifying Chloroplast Biogenesis and Signalling Mutants in Arabidopsis thaliana . In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods in Molecular Biology, vol 684. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-925-3_20

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  • DOI: https://doi.org/10.1007/978-1-60761-925-3_20

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-924-6

  • Online ISBN: 978-1-60761-925-3

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