Abstract
Hydrogen exchange mass spectrometry (HX-MS) is an experimental technique that can be used to examine solvent accessibility and conformational mobility in biological macromolecules. This chapter summarizes studies using HX-MS to examine the regulation of conformation, protein mobility, and ligand binding to MAP kinases. We describe the planning and design of HX-MS experiments, strategies for data analysis and interpretation, and available software.
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Sours, K.M., Ahn, N.G. (2010). Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry. In: Seger, R. (eds) MAP Kinase Signaling Protocols. Methods in Molecular Biology, vol 661. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-795-2_14
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DOI: https://doi.org/10.1007/978-1-60761-795-2_14
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