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Engineered Zinc Finger Proteins pp 457–477Cite as

Crystallization of Zinc Finger Proteins Bound to DNA

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 649))

Abstract

A method is presented for determining conditions for the cocrystallization of zinc finger proteins with DNA. The method describes steps beginning with protein expression, through purification, design of DNA for cocrystallization, and the conditions to screen for cocrystallization.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ, USA

    Nancy C. Horton & Chad K. Park

Authors
  1. Nancy C. Horton
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  2. Chad K. Park
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Editor information

Editors and Affiliations

  1. School of Molecular &, Microbial Biosciences, University of Sydney, Cnr. Butlin Ave. & Maze Cres., Sydney, 2006, New South Wales, Australia

    Joel P. Mackay

  2. Dept. Pharmacology & Genome Center, University of California, Davis, E. Health Sciences Drive 451, Davis, 95616, California, USA

    David J. Segal

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Horton, N.C., Park, C.K. (2010). Crystallization of Zinc Finger Proteins Bound to DNA. In: Mackay, J., Segal, D. (eds) Engineered Zinc Finger Proteins. Methods in Molecular Biology, vol 649. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-753-2_28

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  • DOI: https://doi.org/10.1007/978-1-60761-753-2_28

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-752-5

  • Online ISBN: 978-1-60761-753-2

  • eBook Packages: Springer Protocols

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