Abstract
Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
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References
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Acknowledgments
Studies reported in this article were in part supported by grants from NIH (5PO1CA82834-05, 5PO1AR048818-05, 2R01GM32010, 5R01AR049069). Core resources supported by the Diabetes Endocrinology Research Center grant DK32520 were also used.
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Zaidi, S.K. et al. (2010). Subnuclear Localization and Intranuclear Trafficking of Transcription Factors. In: Higgins, P. (eds) Transcription Factors. Methods in Molecular Biology, vol 647. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-738-9_4
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DOI: https://doi.org/10.1007/978-1-60761-738-9_4
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