Abstract
The combination of surface plasmon resonance (SPR) and mass spectrometry (MS) creates a comprehensive protein investigation approach wherein SPR is employed for protein quantification and MS is utilized to structurally characterize the proteins. In such, MS utterly complements the SPR detection and reveals intrinsic protein structural modifications that go unregistered via the SPR detection. Protein complexes and non-specific binding can also be delineated via the SPR-MS approach. Described here are the protocols and know-how for successful and reproducible integration of SPR and MS. The individual steps of the entire SPR-MS process are illustrated via an example showing analysis of myoglobin from human plasma.
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Acknowledgments
This publication was supported in part by grant number 5 R44 CA82079-04 from the National Institutes of Health. Its contents are solely the responsibility of the author and do not necessarily represent the official views of the National Institutes of Health.
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Nedelkov, D. (2010). Integration of SPR Biosensors with Mass Spectrometry (SPR-MS). In: Mol, N., Fischer, M. (eds) Surface Plasmon Resonance. Methods in Molecular Biology, vol 627. Humana Press. https://doi.org/10.1007/978-1-60761-670-2_18
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DOI: https://doi.org/10.1007/978-1-60761-670-2_18
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