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Integration of SPR Biosensors with Mass Spectrometry (SPR-MS)

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Book cover Surface Plasmon Resonance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 627))

Abstract

The combination of surface plasmon resonance (SPR) and mass spectrometry (MS) creates a comprehensive protein investigation approach wherein SPR is employed for protein quantification and MS is utilized to structurally characterize the proteins. In such, MS utterly complements the SPR detection and reveals intrinsic protein structural modifications that go unregistered via the SPR detection. Protein complexes and non-specific binding can also be delineated via the SPR-MS approach. Described here are the protocols and know-how for successful and reproducible integration of SPR and MS. The individual steps of the entire SPR-MS process are illustrated via an example showing analysis of myoglobin from human plasma.

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Acknowledgments

This publication was supported in part by grant number 5 R44 CA82079-04 from the National Institutes of Health. Its contents are solely the responsibility of the author and do not necessarily represent the official views of the National Institutes of Health.

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Authors and Affiliations

  1. Intrinsic Bioprobes Inc., Tempe, AZ, USA

    Dobrin Nedelkov

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  1. Dobrin Nedelkov
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Editor information

Editors and Affiliations

  1. Dept. Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, Utrecht, 3585 CA, Netherlands

    Nico J. Mol

  2. Dept. Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, Utrecht, 3585 CA, Netherlands

    Marcel J. E. Fischer

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Nedelkov, D. (2010). Integration of SPR Biosensors with Mass Spectrometry (SPR-MS). In: Mol, N., Fischer, M. (eds) Surface Plasmon Resonance. Methods in Molecular Biology, vol 627. Humana Press. https://doi.org/10.1007/978-1-60761-670-2_18

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  • DOI: https://doi.org/10.1007/978-1-60761-670-2_18

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-669-6

  • Online ISBN: 978-1-60761-670-2

  • eBook Packages: Springer Protocols

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