Abstract
Fluorescently labeled carbon nanotube probes (CNTP) are prepared by derivatizing oxidized (o)-MWNTs with a fluorescein dye. Capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) detection is used to separate and detect CNTP in multidrug-resistant cells (K562A) and the parent cells (K562S). CE-LIF and flow cytometry investigation reveal that the CNTP can traverse the membranes in both cell lines without being pumped out by P-glycoprotein. The CE-LIF method is also useful for quantitative analysis of CNT in single cells, enabling drug delivery and multidrug resistance (MDR) studies. Moreover, toward quantifying the intracellular uptake of oxidized (o)-SWNTs with anchored Rhodamine123 (Rho123), fluorescence-quenching of Rho123 is measured by micellar electrokinetic chromatography coupled with LIF detection. Enhanced uptake of Rho123 in multidrug-resistant leukemia cells can be achieved with the aid of the o-SWNTs carriers. Besides being able to overcome MDR, o-SWNTs are shown to be excellent intracellular carriers possessing large adsorption capacity and prolonged release ability. Finally, it is demonstrated that o-SWNTs are safe for biological applications at concentrations of up to 40 µg/mL.
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Acknowledgments
Financial support from the National Natural Sciences Foundation of China (No. 20520120220), the China State Key Basic Research Program Grants (2003CB716002), and the Knowledge Innovation Program of DICP to H.Z. are gratefully acknowledged.
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Li, R., Zou, H., Xiao, H., Wu, R. (2010). Carbon Nanotubes as Intracellular Carriers for Multidrug Resistant Cells Studied by Capillary Electrophoresis–Laser–Induced Fluorescence. In: Balasubramanian, K., Burghard, M. (eds) Carbon Nanotubes. Methods in Molecular Biology, vol 625. Humana Press. https://doi.org/10.1007/978-1-60761-579-8_13
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DOI: https://doi.org/10.1007/978-1-60761-579-8_13
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