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Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells

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Lentivirus Gene Engineering Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 614))

Abstract

Human fetal mesenchymal stem cells (hfMSC) demonstrate extensive expansion and differentiation capacities and are hence being studied for use in stem cell therapeutics, including gene delivery. With advanced prenatal diagnosis, fetal gene therapy represents an additional avenue for the treatment of inherited deficiencies. We have recently demonstrated harvesting of first-trimester fMSC from fetal blood for ex vivo genetic engineering to introduce genes of interest, and finally intra-uterine transplantation (IUT) of these cells to the fetus. Here we discuss methods in the harvesting of hfMSC, lentiviral transduction to introduce genes of interest, and in vitro methods to characterise transgene expression.

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Author information

Authors and Affiliations

  1. Experimental Fetal Medicine Group, Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    Mark S. K. Chong & Jerry Chan

Authors
  1. Mark S. K. Chong
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  2. Jerry Chan
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Corresponding author

Correspondence to Jerry Chan .

Editor information

Editors and Affiliations

  1. Div. Patogenesi dei Retrovirus, Centro Nazionale AIDS, Viale Regina Elena 299, Roma, 00161, Italy

    Maurizio Federico

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Chong, M.S.K., Chan, J. (2010). Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells. In: Federico, M. (eds) Lentivirus Gene Engineering Protocols. Methods in Molecular Biology, vol 614. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-533-0_9

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  • DOI: https://doi.org/10.1007/978-1-60761-533-0_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-532-3

  • Online ISBN: 978-1-60761-533-0

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