Summary
Ethanol production by fermentation of lignocellulosic biomass-derived sugars involves a fairly ancient art and an ever-evolving science. Production of ethanol from lignocellulosic biomass is not avant-garde, and wood ethanol plants have been in existence since at least 1915. Most current ethanol production relies on starch- and sugar-based crops as the substrate; however, limitations of these materials and competing value for human and animal feeds is renewing interest in lignocellulose conversion. Herein, we describe methods for both simultaneous saccharification and fermentation (SSF) and a similar but separate process for partial saccharification and cofermentation (PSCF) of lignocellulosic biomass for ethanol production using yeasts or pentose-fermenting engineered bacteria. These methods are applicable for small-scale preliminary evaluations of ethanol production from a variety of biomass sources.
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Acknowledgments
We would like to thank undergraduate student researchers in the Peterson lab, Brian Gardner, and Sean Suggs for their excellent fermentation help. The authors also acknowledge the assistance of Patricia O’Bryan at the USDA in Peoria for her assistance with the small-volume fermentation procedures. This work was supported, in part, by funding to JDP from the Traditional Industries Program (TIP3) of Georgia.
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Doran-Peterson, J., Jangid, A., Brandon, S.K., DeCrescenzo-Henriksen, E., Dien, B., Ingram, L.O. (2009). Simultaneous Saccharification and Fermentation and Partial Saccharification and Co-Fermentation of Lignocellulosic Biomass for Ethanol Production. In: Mielenz, J. (eds) Biofuels. Methods in Molecular Biology, vol 581. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-214-8_17
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