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Immunofluorescence Analysis of Human Spermatocytes

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Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 558))

Abstract

New immunofluorescence techniques allow visual identification of human cells in various stages of meiotic prophase. Antibodies to the synaptonemal complex, the centromere and sites of recombination allow these stages of meiotic prophase to be identified. The progress of chromosome synapsis, recombination and associated phenomena such as interference can be studied in normal men, translocation heterozygotes and men with infertility problems. This has greatly stimulated research in human meiosis, leading to many exciting studies on the mechanisms underlying recombination and the generation of chromosome abnormalities.

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Author information

Authors and Affiliations

  1. Department of Medical Genetics, University of Calgary, Calgary, Alberta, Canada

    Evelyn Ko & Renée H. Martin

Authors
  1. Evelyn Ko
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  2. Renée H. Martin
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Editor information

Editors and Affiliations

  1. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, York Ave. 1275, New York, 10021-6007, U.S.A.

    Scott Keeney

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Ko, E., Martin, R.H. (2009). Immunofluorescence Analysis of Human Spermatocytes. In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 558. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-103-5_23

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  • DOI: https://doi.org/10.1007/978-1-60761-103-5_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-102-8

  • Online ISBN: 978-1-60761-103-5

  • eBook Packages: Springer Protocols

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