Abstract
Genetic manipulation of adult hippocampal neural progenitor cells is a useful technique for exploring gene function through gain of function and loss of function mutations or RNAi. Furthermore, the introduction of new genes can “re-program” progenitor cell behavior to force a desired lineage in signaling environments that are not normally permissive for that cell fate. Additionally, by using a systems biology approach, neural progenitors can even be taught new behaviors and responses to signaling. In this chapter, we describe protocols for retroviral and lentiviral packaging and transduction of progenitors. These techniques are important for studying the role of various genes in progenitor fate choice.
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Acknowledgment
We acknowledge Matthew Robertson for critical reading of the manuscript. This work was funded by an NSF Graduate Research Fellowship (to JP), a California Institute for Regenerative Medicine Training Grant (T1-00007), and NSF BES-0629202.
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Peltier, J., Schaffer, D.V. (2010). Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors. In: Conboy, I., Schaffer, D., Barcellos-Hoff, M., Li, S. (eds) Protocols for Adult Stem Cells. Methods in Molecular Biology™, vol 621. Humana Press. https://doi.org/10.1007/978-1-60761-063-2_7
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DOI: https://doi.org/10.1007/978-1-60761-063-2_7
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