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Delivery of RNA Interference to Peripheral Neurons In Vivo Using Herpes Simplex Virus

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Analgesia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 617))

Abstract

RNA interference (RNAi) has become a powerful tool for modulating gene expression. While delivery of small interfering RNAs (siRNAs) has achieved silencing of pain-related genes in various animal models of nociception, delivery of short-hairpin RNA (shRNA) or artificial miRNA (miRNA) to dorsal root ganglia (DRG) has proven particularly challenging. This chapter describes a highly efficient method for in vivo gene silencing in sensory neurons using replication-defective vectors based on herpes simplex virus (HSV). This method can be utilised to obtain a better understanding of gene function, validate novel gene targets in drug discovery and potentially develop new RNAi-mediated approaches to achieve analgesia.

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Authors and Affiliations

  1. BioVex Inc., Woburn, MA, USA

    Anna-Maria Anesti

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  1. Anna-Maria Anesti
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Correspondence to Anna-Maria Anesti .

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Editors and Affiliations

  1. Dept. Pathology, Monmouth Medical Center, Second Ave. 300, Long Branch, 07750, USA

    Arpad Szallasi

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Anesti, AM. (2010). Delivery of RNA Interference to Peripheral Neurons In Vivo Using Herpes Simplex Virus. In: Szallasi, A. (eds) Analgesia. Methods in Molecular Biology, vol 617. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-323-7_26

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  • DOI: https://doi.org/10.1007/978-1-60327-323-7_26

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-322-0

  • Online ISBN: 978-1-60327-323-7

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