Abstract
Many molecular genetic studies of human diseases involve determining the genotypes for single nucleotide polymorphisms. This chapter summarises a number of different techniques for the single nucleotide polymorphism genotyping which can be applied to MMP genes. The chapter also provides a protocol and technical notes for the 5ʹ-nuclease assay, one of the most commonly used genotyping techniques.
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Acknowledgement
The authors thank the support of European Union Framework 6 (grant number LSHCCT-2003-503297).
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Laxton, R., Ye, S. (2010). Single Nucleotide Polymorphism Genotyping in MMP Genes: The 5ʹ Nuclease Assay. In: Clark, I. (eds) Matrix Metalloproteinase Protocols. Methods in Molecular Biology, vol 622. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-299-5_13
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DOI: https://doi.org/10.1007/978-1-60327-299-5_13
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