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Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay

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Bacteriophages

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 501))

Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

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Author information

Authors and Affiliations

  1. Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada

    Andrew M. Kropinski & Erika Lingohr

  2. Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada

    Andrew M. Kropinski

  3. Department of Microbiology and Immunology, Queen’s University, Kingston, Ontario, Canada

    Andrew M. Kropinski

  4. Pro-Lab Diagnostics, Richmond Hill, Ontario, Canada

    Amanda Mazzocco & Thomas E. Waddell

  5. Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, Guelph, Ontario, Canada

    Roger P. Johnson

Authors
  1. Andrew M. Kropinski
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  2. Amanda Mazzocco
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  3. Thomas E. Waddell
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  4. Erika Lingohr
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  5. Roger P. Johnson
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Editor information

Editors and Affiliations

  1. University of Leicester, Leicester, UK

    Martha R.J. Clokie

  2. Public Health Agency of Canada, Guelph, Ontario, Canada

    Andrew M. Kropinski

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Kropinski, A.M., Mazzocco, A., Waddell, T.E., Lingohr, E., Johnson, R.P. (2009). Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay. In: Clokie, M.R., Kropinski, A.M. (eds) Bacteriophages. Methods in Molecular Biology™, vol 501. Humana Press. https://doi.org/10.1007/978-1-60327-164-6_7

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  • DOI: https://doi.org/10.1007/978-1-60327-164-6_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-682-5

  • Online ISBN: 978-1-60327-164-6

  • eBook Packages: Springer Protocols

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