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Apoptosis pp 343–356Cite as

Detection of Cell Death in Drosophila

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 559))

Summary

Drosophila is a powerful model system for the identification of cell death genes and understanding the role of cell death in development. In this chapter, we describe three methods typically used for the detection of cell death in Drosophila. The TUNEL and acridine orange methods are used to detect dead or dying cells in a variety of tissues. We focus on methods for the embryo and the ovary, but these techniques can be used on other tissues as well. The third method is the detection of genetic interactions by expressing cell death genes in the Drosophila eye.

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Acknowledgments

We thank Daniela Drummond-Barbosa and current and previous lab members for help in devising these protocols. KM is supported by the NIH R01 GM60574.

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Authors and Affiliations

  1. Department of Biology, Boston University, Boston, MA, USA

    Kimberly McCall, Jeanne S. Peterson & Tracy L. Pritchett

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  1. Kimberly McCall
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  2. Jeanne S. Peterson
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  3. Tracy L. Pritchett
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Editors and Affiliations

  1. Boston Biomedical Research Institute, Grove Street 64, Watertown, 02472, U.S.A.

    Peter Erhardt

  2. Boston Biomedical Research Institute, Grove Street 64, Watertown, 02472, U.S.A.

    Ambrus Toth

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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McCall, K., Peterson, J.S., Pritchett, T.L. (2009). Detection of Cell Death in Drosophila . In: Erhardt, P., Toth, A. (eds) Apoptosis. Methods in Molecular Biology, vol 559. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-017-5_24

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  • DOI: https://doi.org/10.1007/978-1-60327-017-5_24

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-016-8

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