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Protein Blotting and Detection pp 533–543Cite as

Improved Protein Detection Using Cold Microwave Technology

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 536))

Summary

Protein screening/detection is an essential tool in many laboratories. Owing to the relatively large time investments that are required by standard protocols, the development of methods with higher throughput while maintaining an at least comparable signal-to-noise ratio would be highly beneficial to many researchers. This chapter describes how cold microwave technology can be used to enhance the rate of molecular interactions and provides protocols for dot blots, western blots, and ELISA procedures permitting a completion of all incubation steps (blocking and antibody steps) within 45 min.

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Author information

Authors and Affiliations

  1. Microscopy and Imaging Center, Department of Biology and Department of Biochemistry and Biophysics, BSBW 119, Texas A&M University, College Station, TX, 77843-2257, USA

    Aaron G. Smith, Jyothi Jayaram, Carol B. Johnson, E. Ann Ellis, Stanislav Vitha, Ellen W. Collisson & Andreas Holzenburg

Authors
  1. Aaron G. Smith
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  2. Jyothi Jayaram
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  3. Carol B. Johnson
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  4. E. Ann Ellis
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  5. Stanislav Vitha
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  6. Ellen W. Collisson
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  7. Andreas Holzenburg
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Corresponding author

Correspondence to Andreas Holzenburg .

Editor information

Editors and Affiliations

  1. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    R. Hal Scofield

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Cite this protocol

Smith, A.G. et al. (2009). Improved Protein Detection Using Cold Microwave Technology. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_54

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  • DOI: https://doi.org/10.1007/978-1-59745-542-8_54

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-73-2

  • Online ISBN: 978-1-59745-542-8

  • eBook Packages: Springer Protocols

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