Summary
DNA microarrays can be used to detect polymorphic loci in addition to identifying genes or regions that are absent within a genome. A survey such as this offers greater insight into the level of diversification within a species or population, which is useful in organisms that have nearidentical genomic content but differ in phenotype. The identification of such variable loci can then lead to the characterization of genes linked to unique biological attributes. Here, we describe a competitive hybridization assay using DNA microarrays as a comparative genomics tool to identify nucleotide polymorphisms among closely related strains of Chlamydia trachomatis.
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References
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Acknowledgments
We would like to thank Dr. Eric M. Nicholson for his critical evaluation regarding the manuscript. We would also like to thank Dr. Gary Schoolnik and the members of his lab for their technical assistance with the microarray procedures described here. This work was performed in the laboratory of Dr. Richard S. Stephens, and we are grateful the support he provided. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.
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Brunelle, B.W., Nicholson, T.L. (2007). Microarray-Based Approach for Genome-Wide Survey of Nucleotide Polymorphisms. In: Bergman, N.H. (eds) Comparative Genomics. Methods In Molecular Biology™, vol 396. Humana Press. https://doi.org/10.1007/978-1-59745-515-2_17
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DOI: https://doi.org/10.1007/978-1-59745-515-2_17
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