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Peptide and Protein Ion/Ion Reactions in Electrodynamic Ion Traps: Tools and Methods

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Mass Spectrometry of Proteins and Peptides

Part of the book series: Methods In Molecular Biology ((MIMB,volume 492))

Summary

Gas-phase reactions of oppositely charged ions can play important roles in the analysis of peptides and proteins. Electrospray ionization (ESI) can yield multiply charged versions of gaseous peptide and protein ions that can react with oppositely charged ions via several distinct mechanisms. Reagent ions that react via proton transfer can be used to facilitate protein mixture analysis and the mass assignment of product ions in a tandem mass spectrometry experiment. Proton transfer reactions can also be used to concentrate protein ion signals into one or two charge states and can be used to charge state purify a precursor ion population for subsequent dissociation. Electron transfer reactions have been shown to lead to fragmentation of gaseous protonated peptides and proteins. The extent of sequence information available from an electron transfer reaction is often greater than that obtained via conventional collision-induced dissociation. Electron transfer dissociation is particularly useful in probing the structures of polypeptides with labile posttranslational modifications. We summarize here the tools and general methods for conducting ion/ion reaction studies with emphasis on electrodynamic ion traps as the reaction vessels.

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Acknowledgments

This work was supported by the National Institutes of Health, Institute of General Medical Sciences, under Grant GM 45372.

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Authors and Affiliations

  1. Department of Chemistry, Purdue University, West Lafayette, IN, USA

    Scott A. McLuckey

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  1. Scott A. McLuckey
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Editors and Affiliations

  1. Fundamental & Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA

    Mary S. Lipton

  2. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA

    Ljiljana Paša-Tolic

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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McLuckey, S.A. (2009). Peptide and Protein Ion/Ion Reactions in Electrodynamic Ion Traps: Tools and Methods. In: Lipton, M.S., Paša-Tolic, L. (eds) Mass Spectrometry of Proteins and Peptides. Methods In Molecular Biology, vol 492. Humana Press. https://doi.org/10.1007/978-1-59745-493-3_24

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  • DOI: https://doi.org/10.1007/978-1-59745-493-3_24

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-48-0

  • Online ISBN: 978-1-59745-493-3

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