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The Minipig as an Animal Model in Biomedical Stem Cell Research

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Sourcebook of Models for Biomedical Research

Abstract

Pigs and miniature pigs are steadily gaining importance as large animal models in the field of regenerative medicine, including stem cell research. With their size, organ capacity, and physiology resembling in several aspects that of humans, pigs are well suited for preclinical experiments and long-term safety studies. In this chapter, we summarize our experience with the isolation and culture of several somatic stem cell populations from fetal and adult pig tissue and briefly review their potential usefulness in future stem cell-based therapies. We also provide protocols for the isolation of fetal porcine neural stem cells (NSCs), adult bone marrow mesenchymal stem cells (MSCs), and epidermal progenitor cells (EPCs) from adult hair follicles.

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Author information

Authors and Affiliations

  1. Department of Reproductive and Developmental Biology, Institute of Animal Physiology and Genetics, The Academy of Sciences of The Czech Republic, Libechov, Czech Republic

    Petr Vodicka MSC

  2. Institute of Animal Physiology and Genetics, The Academy of Sciences of the Czech Republic, Libechov, Czech Republic

    Jana Hlucilova DVM, Jiri Klima PhD & Jan Motlik DVM, PhD, DSC

  3. Center for Cell Therapy and Tissue Repair, Charles University, Praha, Czech Republic

    Jana Hlucilova DVM, Jiri Klima PhD & Jan Motlik DVM, PhD, DSC

  4. College of Veterinary Medicine, Iowa State University, Ames, IA

    Radek Prochazka DVM, PhD, Jitka Ourednik PhD & Vaclav Ourednik PhD

Authors
  1. Petr Vodicka MSC
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  2. Jana Hlucilova DVM
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  3. Jiri Klima PhD
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  4. Radek Prochazka DVM, PhD
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  5. Jitka Ourednik PhD
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  6. Vaclav Ourednik PhD
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  7. Jan Motlik DVM, PhD, DSC
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Editor information

Editors and Affiliations

  1. Oregon National Primate Research Center, Beaverton, OR

    P. Michael Conn PhD (Associate Director and Senior Scientist, Professor of Physiology and Pharmacology, Professor of Cell and Developmental Biology) (Associate Director and Senior Scientist, Professor of Physiology and Pharmacology, Professor of Cell and Developmental Biology)

  2. Oregon Health and Science University, Portland, OR

    P. Michael Conn PhD (Associate Director and Senior Scientist, Professor of Physiology and Pharmacology, Professor of Cell and Developmental Biology) (Associate Director and Senior Scientist, Professor of Physiology and Pharmacology, Professor of Cell and Developmental Biology)

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© 2008 Humana Press Inc., Totowa, NJ

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Vodicka, P. et al. (2008). The Minipig as an Animal Model in Biomedical Stem Cell Research. In: Conn, P.M. (eds) Sourcebook of Models for Biomedical Research. Humana Press. https://doi.org/10.1007/978-1-59745-285-4_27

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  • DOI: https://doi.org/10.1007/978-1-59745-285-4_27

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-933-8

  • Online ISBN: 978-1-59745-285-4

  • eBook Packages: MedicineMedicine (R0)

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