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Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani

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Plant Pathology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 508))

Summary

Many species of fungi have been shown to harbor double-stranded RNA (dsRNA) elements. A single fungal isolate of Rhizoctonia solani may have as many as five different dsRNA elements within them. The presence of specific dsRNA elements influence pathogenicity in host plants.

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References

  1. Castanho, B., and Butler, E.E. (1978) Rhizoctonia decline: a degenerative disease of Rhizoctonia solani. Phytopathology 68, 1505–1510.

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  2. Tavantzis, S.M., Lakshman, D.K., and Liu, C. (2002) Double-stranded RNA elements modulating virulence in Rhizoctonia solani, in Tavantzis, S.M., ed.), dsRNA Genetic Elements Concepts and Applications in Agriculture, Forestry and Medicine. (CRC Press, Boca Raton, FL, pp. 191–211.

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  3. Tavantzis, S.M., and Lakshman, D.K. (1995) Virus-like double-stranded RNA elements and hypovirulence in phytopathogenic fungi, in Kohmoto, K., Singh, U.S., Singh R.P., eds.), Pathogenesis and Host–Parasite Specificity in Plant Disease: Histopathological, Biochemical, Genetic and Molecular Basis, Elsevier (Pergamon) Press, Oxford, pp. 249.––267Vol. III. (

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  4. Liu, C., Lakshman, D.K., and Tavantzis, S.M. (2003) Quinic acid induces hypovirulence and expression of a hypovirulence-associated double-stranded RNA in Rhizoctonia solani. Curr. Genet. 43, 103–111.

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  5. Morris, T.J., and Dodds, J.A. (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69, 854–858.

    Article  CAS  Google Scholar 

  6. Salzman, R.A., Fujita, T., Zhu-Salzman, K., Hasegawa, P.M., and Bressan, R.A. (1999) An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates. Plant Mol. Biol. Rep. 17, 11–17.

    Article  CAS  Google Scholar 

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Charlton, N., Tavantzis, S., Cubeta, M. (2009). Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani . In: Burns, R. (eds) Plant Pathology. Methods in Molecular Biology, vol 508. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-062-1_14

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  • DOI: https://doi.org/10.1007/978-1-59745-062-1_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-799-0

  • Online ISBN: 978-1-59745-062-1

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