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Formation of Amphipathic Amyloid Monolayers from Fungal Hydrophobin Proteins

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Protein Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2073))

Abstract

The fungal hydrophobins are small proteins that are able to self-assemble spontaneously into amphipathic monolayers at hydrophobic:hydrophilic interfaces. These protein monolayers can reverse the wettability of a surface, making them suitable for increasing the biocompatibility of many hydrophobic nanomaterials. One subgroup of this family, the class I hydrophobins, forms monolayers that are composed of extremely robust amyloid-like fibrils, called rodlets. Here, we describe the protocols for the production and purification of recombinant hydrophobins and oxidative refolding to a biologically active, soluble, monomeric form. We describe methods to trigger the self-assembly into the fibrillar rodlet state and techniques to characterize the physicochemical properties of the polymeric forms.

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Acknowledgments

This work was supported by funding from the National Health and Medical Research Council of Australia (CDA402831) and the Australian Research Council (LP0776672, DP0879121 and DP150104227). V. Lo was supported by an Australian Postgraduate Award and S. Ball by a Research Training Program stipend.

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Authors and Affiliations

  1. Discipline of Pharmacology and Sydney Nano, University of Sydney, Sydney, NSW, Australia

    Sarah R. Ball, Chi L. L. Pham, Victor Lo & Margaret Sunde

  2. School of Biological Science, University of Canterbury, Canterbury, New Zealand

    Vanessa K. Morris

  3. School of Life and Environmental Sciences and Sydney Nano, The University of Sydney, Sydney, NSW, Australia

    Ann H. Kwan

Authors
  1. Sarah R. Ball
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  2. Chi L. L. Pham
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  3. Victor Lo
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  4. Vanessa K. Morris
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  5. Ann H. Kwan
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  6. Margaret Sunde
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Corresponding author

Correspondence to Margaret Sunde .

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Editors and Affiliations

  1. School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

    Juliet A. Gerrard

  2. School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

    Laura J. Domigan

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Ball, S.R., Pham, C.L.L., Lo, V., Morris, V.K., Kwan, A.H., Sunde, M. (2020). Formation of Amphipathic Amyloid Monolayers from Fungal Hydrophobin Proteins. In: Gerrard, J., Domigan, L. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 2073. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9869-2_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9868-5

  • Online ISBN: 978-1-4939-9869-2

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