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Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry

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Single Cell Metabolism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2064))

Abstract

In recent years, innovations in mass spectrometry imaging (MSI) have enabled simultaneous detection and mapping of biomolecules and xenobiotics directly from biological tissues and single cells. Matrix-assisted laser desorption ionization (MALDI) has been the most widely embraced MSI technique. However, this technique can exhibit ion suppression effects hindering metabolite coverage and possesses a narrow dynamic range. Nanophotonic platforms, e.g., silicon nanopost array (NAPA) structures, can be used as an alternative for matrix-free imaging of biological tissues. Here, we present a protocol for MSI of large and small adherent cell clusters by laser desorption ionization from NAPA with minimal sample preparation.

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Acknowledgments

Research was sponsored by the U.S. Army Research Office and the Defense Advanced Research Projects Agency and was accomplished under Cooperative Agreement Number W911NF-14-2-0020. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Office, DARPA, or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation hereon.

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Authors and Affiliations

  1. Department of Chemistry, The George Washington University, Washington, DC, USA

    Sylwia A. Stopka & Akos Vertes

Authors
  1. Sylwia A. Stopka
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  2. Akos Vertes
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Correspondence to Sylwia A. Stopka .

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Editors and Affiliations

  1. Waters Corporation, Milford, MA, USA

    Bindesh Shrestha

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Stopka, S.A., Vertes, A. (2020). Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry. In: Shrestha, B. (eds) Single Cell Metabolism. Methods in Molecular Biology, vol 2064. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9831-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9831-9_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9829-6

  • Online ISBN: 978-1-4939-9831-9

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