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Immunophenotyping pp 1–29Cite as

High-Dimensional Immunophenotyping with Fluorescence-Based Cytometry: A Practical Guidebook

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2032))

Abstract

Recent technological advances have greatly diversified the platforms that are available for high-dimensional single-cell immunophenotyping, including mass cytometry, single-cell RNA sequencing, and fluorescent-based flow cytometry. The latter is currently the most commonly used approach, and modern instrumentation allows for the measurement of up to 30 parameters, revealing deep insights into the complexity of the immune system.

Here, we provide a practical guidebook for the successful design and execution of complex fluorescence-based immunophenotyping panels. We address common misconceptions and caveats, and also discuss challenges that are associated with the quality control and analysis of these data sets.

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Acknowledgments

The authors thank all members of the Prlic lab and Dr. Sabine Spath for critical reading of the manuscript. F.M. and A.J.T. are supported by a Marylou scholarship from the International Society for Advancement of Cytometry (ISAC).

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Authors and Affiliations

  1. Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

    Florian Mair

  2. BD Biosciences, La Jolla, CA, USA

    Aaron J. Tyznik

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  1. Florian Mair
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  2. Aaron J. Tyznik
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Corresponding author

Correspondence to Florian Mair .

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Editors and Affiliations

  1. Frederick, MD, USA

    J. Philip McCoy, Jr

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Mair, F., Tyznik, A.J. (2019). High-Dimensional Immunophenotyping with Fluorescence-Based Cytometry: A Practical Guidebook. In: McCoy, Jr, J. (eds) Immunophenotyping. Methods in Molecular Biology, vol 2032. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9650-6_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9650-6_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9649-0

  • Online ISBN: 978-1-4939-9650-6

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