Abstract
Multiple reaction monitoring (MRM) mass spectrometry may be regarded as the gold standard methodology for quantitative mass spectrometry and has been adopted for the analysis of small molecules especially within the pharmaceutical industry. It can also be applied to the analysis of peptides and proteins and to measurement of the basic building blocks of proteins, amino acids. Here we describe the application of MRM mass spectrometry to the measurement of hydroxyproline after acid hydrolysis of various animal tissues. We show that measurement of hydroxyproline provides an accurate and reliable estimate of the collagen content of such tissues and may be a useful indicator of meat tenderness.
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Acknowledgment
This work was supported by the Cooperative Research Centre for Sheep Innovation. The authors thank Dr. David Hopkins of NSW Agriculture, Cowra, NSW, Australia, and Mr. Malcolm Boyce of Murdoch University, WA, Australia, for the supply of the freeze-dried muscle tissue samples used in this study. The authors also thank the Molecular and Cellular Proteomics Facility at The University of Queensland Institute for Molecular Bioscience for access to equipment.
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Colgrave, M.L., Allingham, P.G., Tyrrell, K., Jones, A. (2019). Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen Content. In: Alterman, M. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 2030. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9639-1_4
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DOI: https://doi.org/10.1007/978-1-4939-9639-1_4
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