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Non-Natural Nucleic Acids pp 15–25Cite as

Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites: 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine and 2′-Deoxycytidine

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1973))

Abstract

Formation of adducts to DNA is of great benefit to DNA sequencing and damage detection technology and to enzymology. Here we describe the synthesis and characterization procedures of 18-crown-6 adducts formed to abasic (AP) sites, 8-oxo-7,8-dihydro-2′-deoxyguanosine (OG), and 2′-deoxycytidine (C) residues in DNA oligodeoxynucleotides. These crown ether adducts were used as site-specific modifications to facilitate nanopore technology. The methods described can be readily expanded to attach other suitable primary amines of interest.

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Acknowledgment

This work was supported by grants from the National Institutes of Health, HG005095 and GM093099.

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Authors and Affiliations

  1. Department of Chemistry, University of Utah, Salt Lake City, UT, USA

    Na An, Aaron M. Fleming, Nicole C. Rosecrans, Yi Liao & Cynthia J. Burrows

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  1. Na An
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  2. Aaron M. Fleming
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  3. Nicole C. Rosecrans
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  4. Yi Liao
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  5. Cynthia J. Burrows
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Corresponding author

Correspondence to Cynthia J. Burrows .

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Editors and Affiliations

  1. Department of Chemistry and Biochemistry, Georgia Southern University, Savannah, GA, USA

    Nathaniel Shank

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An, N., Fleming, A.M., Rosecrans, N.C., Liao, Y., Burrows, C.J. (2019). Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites: 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine and 2′-Deoxycytidine. In: Shank, N. (eds) Non-Natural Nucleic Acids. Methods in Molecular Biology, vol 1973. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9216-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-9216-4_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9215-7

  • Online ISBN: 978-1-4939-9216-4

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