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Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset-Induced Pluripotent Stem Cells

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Neural Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1919))

Abstract

Neural stem cells (NSCs) are multipotent and self-renewing precursor cells that give rise to all cell types of the central nervous system (CNS). They can be used for modeling CNS in vitro, for developmental studies and for cell replacement therapies. NSCs can be derived from pluripotent stem cells through differentiation using specific growth factors. Nonhuman primates (NHP) are critical preclinical models for translational research. Induced pluripotent stem cells (iPSCs) can be generated from NHP for the purposes of allogenic or autologous cell replacement studies. Here, we describe the derivation of NSCs from NHP iPSCs.

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Acknowledgments

The authors thank members of the Daadi laboratory for helpful support and suggestions. This work was supported by the Worth Family Fund, the Perry & Ruby Stevens Charitable Foundation and the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation, the NIH primate center base grant (Office of Research Infrastructure Programs/OD P51 OD011133), the National Center for Advancing Translational Sciences, and the National Institutes of Health, through Grant UL1 TR001120.

Disclosures: Dr. Marcel M. Daadi is founder of the biotech company NeoNeuron.

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Authors and Affiliations

  1. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA

    Hyenjong Hong, Gourav Roy-Choudhury, Jeffrey Kim & Marcel M. Daadi

  2. Department of Radiology, Research Imaging Institute, Cell Systems and Anatomy, Long School of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA

    Marcel M. Daadi

Authors
  1. Hyenjong Hong
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  2. Gourav Roy-Choudhury
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  3. Jeffrey Kim
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  4. Marcel M. Daadi
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Corresponding author

Correspondence to Marcel M. Daadi .

Editor information

Editors and Affiliations

  1. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA

    Marcel M. Daadi

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Hong, H., Roy-Choudhury, G., Kim, J., Daadi, M.M. (2019). Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset-Induced Pluripotent Stem Cells. In: Daadi, M. (eds) Neural Stem Cells. Methods in Molecular Biology, vol 1919. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9007-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9007-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9005-4

  • Online ISBN: 978-1-4939-9007-8

  • eBook Packages: Springer Protocols

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