Abstract
Identification of membrane protein interactomes is a key issue to better understand how these molecules carry out their functions. However, protein-protein interactions using conventional interaction assays are particularly challenging for integral membrane proteins, because of their hydrophobic nature. Here we describe the membrane yeast two-hybrid (MbY2H) system, a powerful tool for identifying the interactors of membrane and membrane-associated proteins.
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Acknowledgment
M.V. is supported by a fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche (MESR). Work in C.T.’s lab is funded by the Université Clermont Auvergne, CNRS and INSERM. C.T. would like to thank Aline Probst for critical reading of the manuscript.
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Voisin, M., Vanrobays, E., Tatout, C. (2018). Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System. In: Gundersen, G., Worman, H. (eds) The LINC Complex. Methods in Molecular Biology, vol 1840. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8691-0_16
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DOI: https://doi.org/10.1007/978-1-4939-8691-0_16
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