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Single-Molecule Patterning via DNA Nanostructure Assembly: A Reusable Platform

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DNA Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1811))

Abstract

Here we describe a facile strategy of general applicability for controlling the immobilization of individual nanomoieties on nanopatterned surfaces with single-molecule control. We combine the ability of DNA nanostructures as programmable platforms, with a one-step Focused Ion Beam nanopatterning, to demonstrate the controlled immobilization of DNA origami functionalized with individual quantum dots (QDs) at predesigned positions on glass coverslips and silicon substrates. Remarkably, the platform developed is reusable after a simple cleaning process, and can be designed to display different geometrical arrangements.

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Acknowledgments

D.H. is financially supported by the Chinese Scholarship Council. We further gratefully acknowledge financial support from Queen Mary University of London.

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Authors and Affiliations

  1. School of Biological and Chemical Sciences, Queen Mary University of London, London, UK

    Da Huang, Mark Freeley & Matteo Palma

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  1. Da Huang
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  2. Mark Freeley
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  3. Matteo Palma
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Correspondence to Matteo Palma .

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Editors and Affiliations

  1. Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy

    Giampaolo Zuccheri

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Huang, D., Freeley, M., Palma, M. (2018). Single-Molecule Patterning via DNA Nanostructure Assembly: A Reusable Platform. In: Zuccheri, G. (eds) DNA Nanotechnology. Methods in Molecular Biology, vol 1811. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8582-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-8582-1_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8581-4

  • Online ISBN: 978-1-4939-8582-1

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