Abstract
Protein–small molecule interaction studies provide useful insights into biological processes taking place within the living cell. A special yeast hybrid system, the yeast three-hybrid method, has been developed and used to explore proteins that bind to small molecules, by which means it may be possible to unravel biological processes and dissect function of biological systems. Here we present a protocol employing this method for identifying such binding proteins.
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Wang, Y., Letham, D.S., John, P.C.L., Zhang, R. (2018). Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules. In: Oñate-Sánchez, L. (eds) Two-Hybrid Systems. Methods in Molecular Biology, vol 1794. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7871-7_14
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DOI: https://doi.org/10.1007/978-1-4939-7871-7_14
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7871-7
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