Abstract
Autophagy is a key cellular mechanism involved in the degradation of long-lived proteins and organelles. We and others have previously shown that Schwann cells are able to degrade their own myelin by a form of selective autophagy, or myelinophagy. There is now increasing evidence that myelinophagy could also be aberrantly activated in other demyelinating diseases, including hereditary or inflammatory neuropathies, implicating this pathway in the pathogenesis of these disorders. In this chapter, we describe our protocol to monitor autophagy in peripheral nerves, using the autophagy flux assay. This assay can be useful to compare basal and demyelination-induced autophagy in genetic mice models, or after treatment with specific compounds.
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Acknowledgments
AW is grateful for the support of the Ministerio de Economía y Competitividad–Plan Nacional de I+D+I (Subprograma Ramón y Cajal RYC2010-06901; Proyectos Retos Investigación SAF2015-65360-R; Proyectos ExploraCiencia SAF2015-72416-EXP; Proyectos Europa Excelencia SAF2015-62588-ERC), the BBVA foundation and Ikerbasque Foundation. MP is grateful for the support of the Basque Government of Education fellowship. MT isgrateful for the support of the “Ayudas para contratos predoctorales para la formación de doctores” from the Ministerio de Economía y Competitividad. MVR is grateful for the support of a 2017 Leonardo Grant for Researchers and Cultural Creators, BBVA Foundation. CIBERehd is funded by the Instituto de Salud Carlos III. We thank MINECO for the Severo Ochoa Excellence Accreditation (SEV-2016-0644).
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Tamayo Caro, M., Palomo Irigoyen, M., Pérez Andrés, E., Barreira Manrique, A., Varela Rey, M., Woodhoo, A. (2018). Analyzing Autophagic Flux in Nerve Cultures. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_15
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DOI: https://doi.org/10.1007/978-1-4939-7862-5_15
Publisher Name: Humana Press, New York, NY
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