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Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1773))

Abstract

Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion.

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Notes

  1. 1.

    This period can vary from 24 to 72 h depending on the number of cells attached to the plastic surface (observed under microscope).

  2. 2.

    Using ethanol, there is a risk that the lipids will be eluted from the cells.

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Author information

Authors and Affiliations

  1. Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA

    Jie Li, J. Lowry Curley, Xiying Wu & Jeffrey M. Gimble

  2. LaCell LLC, New Orleans, LA, USA

    Jie Li, J. Lowry Curley, Xiying Wu & Jeffrey M. Gimble

  3. Ubiquitin Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA

    Z. Elizabeth Floyd

  4. Center for Computational and Integrative Biology, Harvard Medical School, Boston, MA, USA

    Yuan Di C. Halvorsen

Authors
  1. Jie Li
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  2. J. Lowry Curley
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  3. Z. Elizabeth Floyd
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  4. Xiying Wu
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  5. Yuan Di C. Halvorsen
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  6. Jeffrey M. Gimble
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Corresponding author

Correspondence to Jeffrey M. Gimble .

Editor information

Editors and Affiliations

  1. Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA

    Bruce A. Bunnell  & Jeffrey M. Gimble  & 

  2. LaCell LLC, New Orleans, LA, USA

    Jeffrey M. Gimble

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Li, J., Curley, J.L., Floyd, Z.E., Wu, X., Halvorsen, Y.D.C., Gimble, J.M. (2018). Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7799-4_13

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7797-0

  • Online ISBN: 978-1-4939-7799-4

  • eBook Packages: Springer Protocols

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