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Identification of Response Elements on Promoters Using Site-Directed Mutagenesis and Chromatin Immunoprecipitation

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Colorectal Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1765))

Abstract

Proximal promoters are located upstream of the transcription start sites of genes, and they contain regulatory sequences on which bind different transcription factors for promoting colorectal cancer progression. Here we describe the comprehensive methodology used previously for the identification and functional characterization of MYC-responsive elements in the integrin α1 subunit (ITGA1) gene using a combination of in silico analysis, site-directed mutagenesis, and chromatin immunoprecipitation.

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References

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Acknowledgments

The original work was supported by the Canadian Institute of Health Research Grants MOP-97836 and MOP-123415. J-FB was the recipient of the Canadian Research Chair in Intestinal Physiopathology. JCC is a scholar of the Fonds de la Recherche du Québec-Santé (FRQS). SB is a recipient of the FRQS postdoctoral fellowship.

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Authors and Affiliations

  1. Cancer Molecular Pathology Section, Laboratory of Pathology, National Cancer Institute, Bethesda, MD, USA

    Salah Boudjadi

  2. Department of Medicine, Faculty of Medicine and Health Science, Université de Sherbrooke, Sherbrooke, QC, Canada

    Julie C. Carrier

  3. Laboratory of Intestinal Physiopathology, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada

    Jean-François Beaulieu

Authors
  1. Salah Boudjadi
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  2. Julie C. Carrier
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  3. Jean-François Beaulieu
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Corresponding author

Correspondence to Salah Boudjadi .

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Editors and Affiliations

  1. Laboratory of Intestinal Physiopathology, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Science, Université de Sherbrooke, Sherbrooke, Québec, Canada

    Jean-François Beaulieu

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Boudjadi, S., Carrier, J.C., Beaulieu, JF. (2018). Identification of Response Elements on Promoters Using Site-Directed Mutagenesis and Chromatin Immunoprecipitation. In: Beaulieu, JF. (eds) Colorectal Cancer. Methods in Molecular Biology, vol 1765. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7765-9_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7765-9_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7764-2

  • Online ISBN: 978-1-4939-7765-9

  • eBook Packages: Springer Protocols

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