Abstract
As a major intracellular degradation pathway, autophagy contributes to nutrient recycling and is indispensable during plant senescence. Here we describe methods used for investigating the autophagic process during leaf senescence. These include transcript analysis of core machinery autophagy genes, immunoblotting of ATG8, and microscopic observation of autophagosome formation.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31401179) and the Fundamental Research Funds for the Central Universities to QG.
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Cui, X., Zheng, J., Zheng, J., Gong, Q. (2018). Study of Autophagy in Plant Senescence. In: Guo, Y. (eds) Plant Senescence. Methods in Molecular Biology, vol 1744. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7672-0_23
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DOI: https://doi.org/10.1007/978-1-4939-7672-0_23
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