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Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures

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Glioblastoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1741))

Abstract

Glioblastoma (GBM), a deadly primary brain malignancy, manifests pronounced radioresistance. Identifying agents that improve the sensitivity of tumor tissue to radiotherapy is critical for improving patient outcomes. The response to ionizing radiation is regulated by both cell-intrinsic and -extrinsic mechanisms. In particular, the tumor microenvironment is known to promote radioresistance in GBM. Therefore, model systems used to test radiosensitizing agents need to take into account the tumor microenvironment. We recently showed that GBM explant cultures represent an adaptable ex vivo platform for rapid and personalized testing of radiosensitizers. These explants preserve the cellular composition and tissue architecture of parental patient tumors and therefore capture the microenvironmental context that critically determines the response to radiotherapy. This chapter focuses on the detailed protocol for testing candidate radiosensitizing agents in GBM explants.

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Author information

Author notes

  1. N. Sumru Bayin, Lin Ma contributed equally to this work.

Authors and Affiliations

  1. Department of Neurosurgery, New York University School of Medicine, New York, NY, USA

    N. Sumru Bayin & Dimitris G. Placantonakis

  2. Kimmel Center for Stem Cell Biology, New York University School of Medicine, New York, NY, USA

    N. Sumru Bayin & Dimitris G. Placantonakis

  3. Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA

    N. Sumru Bayin

  4. Department of Radiation Oncology, University of California, San Francisco, San Francisco, CA, USA

    Lin Ma & Mary Helen Barcellos-Hoff

  5. Perlmutter Cancer Center, New York University School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

  6. Brain Tumor Center, New York University School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

  7. Neuroscience Institute, NYU School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

Authors
  1. N. Sumru Bayin
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  2. Lin Ma
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  3. Dimitris G. Placantonakis
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  4. Mary Helen Barcellos-Hoff
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Corresponding authors

Correspondence to Dimitris G. Placantonakis or Mary Helen Barcellos-Hoff .

Editor information

Editors and Affiliations

  1. Department of Neurosurgery, NYU School of Medicine, New York, New York, USA

    Dimitris G. Placantonakis

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Bayin, N.S., Ma, L., Placantonakis, D.G., Barcellos-Hoff, M.H. (2018). Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures. In: Placantonakis, D. (eds) Glioblastoma. Methods in Molecular Biology, vol 1741. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7659-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7659-1_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7658-4

  • Online ISBN: 978-1-4939-7659-1

  • eBook Packages: Springer Protocols

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