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Manipulation and Measurement of AMPK Activity in Pancreatic Islets

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AMPK

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1732))

Abstract

The role of the energy sensor AMPK-activated protein kinase (AMPK) in the insulin-secreting β-cell remains unclear and a subject of intense research. With this chapter, we aim to provide a detailed description of the methods that our group routinely applies to the study of AMPK function in mouse and human pancreatic islets. Thus, we provide detailed protocols to isolate and/or culture mouse and human islets, to modulate and measure AMPK activity in isolated islets, and to evaluate its impact on islet function.

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Authors and Affiliations

  1. Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology and Metabolism, Imperial College London, London, UK

    Aida Martinez-Sanchez, Marie-Sophie Nguyen-Tu, Isabelle Leclerc & Guy A. Rutter

Authors
  1. Aida Martinez-Sanchez
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  2. Marie-Sophie Nguyen-Tu
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  3. Isabelle Leclerc
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  4. Guy A. Rutter
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Corresponding authors

Correspondence to Aida Martinez-Sanchez or Guy A. Rutter .

Editor information

Editors and Affiliations

  1. Department of Pathology CARIM School for Cardiovascular Diseases, Maastricht University, Maastricht, The Netherlands

    Dietbert Neumann

  2. Department EMD, Inserm, U1016, Institut Cochin, CNRS, UMR8104, Université Paris Descartes, Paris, France

    Benoit Viollet

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Martinez-Sanchez, A., Nguyen-Tu, MS., Leclerc, I., Rutter, G.A. (2018). Manipulation and Measurement of AMPK Activity in Pancreatic Islets. In: Neumann, D., Viollet, B. (eds) AMPK. Methods in Molecular Biology, vol 1732. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7598-3_26

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  • DOI: https://doi.org/10.1007/978-1-4939-7598-3_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7597-6

  • Online ISBN: 978-1-4939-7598-3

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