Abstract
Proteins act as executors for almost all kinds of cellular processes. The majority of proteins achieve their proper functions through interacting with other proteins. Knowing the binding partners of a protein is instrumental for understanding its function. The antibody pull-down method is a powerful and common approach to detect protein-protein interactions. Here, an antibody pull-down protocol is described for detecting protein-protein interactions in fission yeast Schizosaccharomyces pombe.
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Acknowledgments
We would like to thank past and present members of Li lab who contributed to the methods presented here, and thank Dr. Jinpu Yang for reading the manuscript. F.L. is a Pew Scholar in the Biomedical Sciences, supported by The Pew Charitable Trusts. This work was supported by National Institutes of Health grant R01GM106037 (to F.L.)., National Science Foundation grant MCB-1330557 (to F.L.), and the National Natural Science Foundation of China (NSFC) Grant 31728010 (to F.L.).
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Dong, Q., Li, F. (2018). Antibody Pull-Down Experiments in Fission Yeast. In: Singleton, T. (eds) Schizosaccharomyces pombe. Methods in Molecular Biology, vol 1721. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7546-4_11
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DOI: https://doi.org/10.1007/978-1-4939-7546-4_11
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Online ISBN: 978-1-4939-7546-4
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